Pursuing efficient workflow
The ECLIPSE Si has been developed with the primary goal of reducing fatigue during microscope usage. The ECLIPSE Si eliminates unnecessary adjustments and enables efficient and comfortable operation. The ergonomic design also enables natural posture, even when carrying out repetitive tasks.
Maintaining comfortable brightness when switching magnifications
Objectives with different magnifications transmit light to varying degrees. Therefore, light intensity must be adjusted every time the user changes the objective. In addition, when switching from high to low magnification objectives, the sudden increase in brightness often causes eye strain. The ECLIPSE Si features the intelligent Light Intensity Management (LIM) which automatically remembers and sets the light intensity level for each objective. With the ECLIPSE Si, users can increase comfort and save time even when the routine requires frequent magnification changes.
Low stage for effortless slide replacement
The height of ECLIPSE Si stage is 135 mm, which is about 50 mm lower than our conventional microscopes. The lower stage design reduces the range of motion required to exchange specimen slides, and in turn this can reduce arm and shoulder fatigue. Since the position of the stage movement knob is also lower, different areas on the specimen slide can be easily explored while resting your hands on the table. The lever for opening and closing the specimen holder has also been designed to be ergonomic with an easy-to-operate size and shape. Furthermore, the ECLIPSE Si features a 30% smaller stage compared to our conventional microscopes in order to optimise slide replacement.
Enables natural posture to be maintained throughout the entire microscope workflow
The inclination angle of the eyepiece tube is 45 degrees, which enables observation through the eyepieces while maintaining a natural posture. The low stage design also allows you to seamlessly switch from looking through the eyepieces to checking the slide placement on the stage without having to adjust your posture. An optional eye-level riser is also available to further tailor the height of the eyepieces.
Worry free focusing thanks to vertical stop
The ECLIPSE Si is equipped with a stopper that can be used to set the upper limit of the stage height. The stage stops at the set height even when the focus knob is turned, thereby eliminating the risk of over-focusing and breaking the slides or damaging the objectives. Specimen exchange and focusing can be performed with confidence, without worrying about the stage height.
Pursuing stress-free ease of use
Adjusting focus and moving the stage with one hand
The coarse and fine focus knobs are located on both sides of the microscope, making it possible to focus with either hand. In addition, the stage handle is positioned close to the focus knob, allowing users to easily adjust both the stage position and focus with the same hand. With the stage movement and focus controlled by the same hand, the other hand can be dedicated to rotating the nosepiece or replacing the slides. These features provide an efficient workflow even when examining a large number of specimen slides.
Status display
The illumination brightness is shown in a bar graph on the LCD. You can check the magnification at a glance while maintaining your observation posture.
Changing magnifications comfortably
The reversed-type nosepiece provides easy access and visibility to the objective in use. The position of the nosepiece is low to reduce strain on the arm when frequent magnification changes are required. The nosepiece features an easy grip for smooth rotation, and accommodates up to five objectives to provide a wide range of magnifications.
Automatically turning off illumination after a certain period of time
The ECLIPSE Si is equipped with an ECO mode which automatically turns off the illumination after a certain period of inactivity. The length of the inactivity period is adjustable. With ECO mode, the ECLIPSE Si helps you save power without any effort.
Compatible with a Wide Variety of Observation Methods
Brightfield observation
High-quality images can be acquired with bright, uniform illumination over the entire field of view, using objectives with superior image flatness and excellent chromatic aberration correction.
Phase contrast observation
Combining of a phase condenser and phase contrast objectives enables observation of colorless and transparent specimens with high contrast, without staining or labeling the specimens with dyes. A standard Abbe condenser, with a PH slider inserted, also allows phase contrast observation.
Darkfield observation
By inserting a slider for darkfield microscopy into the condenser slot and using oblique illumination, light scattered by specimens can be visualized. This method is effective for observation of unstained specimens such as live bacteria and examination of colloidal particles.
Simple polarizing observation
By attaching a polarizer to the field lens and an analyzer to the eyepiece tube mount, simple polarizing observation can be performed. The polarization state can be adjusted by turning the polarizer.
Diascopic fluorescence observation
Nikon has developed a unique diascopic fluorescence illumination method that enables easy fluorescence observation without attaching dedicated episcopic illuminator and fluorescence observation equipment. By simply inserting an EX filter slider into the condenser slot and a BA filter slider into the nosepiece slot, fluorescence observation of specimens expressing GFP or stained with fluorescent dyes such as FITC and Alexa 488 can be performed. 
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