Flexible system expandability to meet diverse needs 

Ni series accessories are segmented by function, allowing you to select required units and flexibly combine them to create lean and effective system configurations. 

Confocal imaging 

Combining the Ni-E’s high-precision Z-focus mechanism with Nikon’s confocal microscope system allows high-resolution, high-S/N-ratio imaging of 3D structures of organs and cells. The Ni-E has a highly stable structure suitable for mounting a confocal scanner and features a wide luminous flux that enables bright confocal imaging. 

Simultaneous multichannel imaging 

The Ni’s flexible stratum structure allows the back camera port unit and the epi-fluorescence attachment to be mounted at the same time, enabling simultaneous image acquisition of two different wavelengths with each camera. This enables the capture of high-resolution images in the entire frame for each wavelength without dividing the CCD chip. The use of individual cameras for acquisition allows the user to tailor acquisition parameters for each channel independently, allowing acquisition of high-sensitivity FRET images. 

Imaging of fluorescent-labeled specimens 

In combination with a motorized nosepiece and a motorized epi-fluorescence cube turret, each unit is automatically controlled according to the camera settings such as exposure time, camera gain, and time interval, allowing fast, efficient image acquisition. 

The motorized epi-fluorescence cube turret shutter, which helps to reduce photobleaching of specimens, is easily operated with a convenient Ni-L/Ni-U remote control pad. 

Automated imaging of pathological specimens

With the Ni-E, optimal brightness can be automatically adjusted with objective changeover, eliminating the need for manual adjustment. By controlling the optical zooming of the motorized DSC zooming port for quadrocular tube, it is possible to capture images with the desired angle of field while maintaining the image quality. 

Ni-E provides fully motorized microscope control. 

Microscope operations related to observation can be automatically controlled as previously set. The Ni-E is a fully motorized model that is efficient for experiments requiring comprehensive control of various devices, such as photoactivation devices and confocal systems. 

Automatic adjustment with objective changeover (Ni-E)  Condenser, aperture, field diaphragm and ND filter are automatically set to optimal positions according to the objective magnification in use. In addition, XYZ stage travel distance per handle rotation and parfocal distance deviation correction are automatically adjusted.	Reproducing observation conditions (Ni-E)  Selected observation conditions can be designated to individual buttons, enabling changes to be made at the push of a button. This is particularly convenient when reproducing specific observation conditions.	High-precision motorized focusing (Ni-E)  High-precision Z-focus provides accurate Z-position information required for use with confocal laser microscopes and supports automated Z-series acquisition. Individual coarse and fine focus knobs enhance ease of operation.

High optical performance 

Nano Crystal Coat technology 

Without nano crystal coat (left) with nano crystal coat (right) 

Employed for the first time in microscope objectives, this anti-reflective coating consists of nanometer-size particles. It is based on semiconductor manufacturing technology and is also used for Nikon camera lenses. The coating's coarse structure and uniform arrangement of particles in a spongy construction result in extremely low reflective indices. 

Uniformly bright illumination 

A built-in “fly-eye” lens ensures uniform and bright illumination of the field of view, from edge-to-edge, at any magnification. 

Fluorescence noise elimination 

Nikon's proprietary noise terminator mechanism is employed in the epi-fluorescence cube turret and filter cubes. The S/N ratio is dramatically improved by eliminating stray light in the filter cubes, allowing images with weak fluorescent signals to be captured with high contrast and brightness. 

High color rendering LED light source 

The high color rendering LED light source built-into the Ni-L provides natural color reproducibility comparable to a halogen light source, as well as light uniformity, long life, and other advantages associated with LEDs, making it effective for observation of pathological specimens.